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Simultaneous production of biopesticide and alkaline proteases by Bacillus thuringiensis u

E-coli and spinach? My son worked at a biopesticide research lab in 1998-1999 as an assistant researcher. He has illness/diseases because of this employment. He has gotten the "shaft" in all areas of trying to expose this. The company "discovers" microbes from all over the world. Why do we innoculate to visit another country when microbes are brought into the U.S.?
Simultaneous production of biopesticide and alkaline proteases by Bacillus thuringiensis using SEWAGE SLUDGE as a raw material.

* Tyagi RD,
* Sikati Foko V,
* Barnabe S,
* Vidyarthi AS,
* Valero JR,
* Surampalli RY.

INRS-Eau, 2700 rue Einstein, Sainte-Foy, Quebec, Canada G1 V 4C7.  tyagi@inrs-eau.uquebec.ca

The simultaneous production of Bacillus thuringiensis (Bt) based biopesticide and proteases was studied using synthetic medium and wastewater sludge as a raw material. The studies were conducted in shake flask and computer controlled 15-L capacity fermentors. Measuring viable cell and spore counts, entomotoxicity and protease activity monitored the progress of the biopesticide production process. A higher viable cell count and spore count was observed in synthetic Soya medium, however, higher entomotoxicity and protease activity were observed in wastewater sludge medium. Thus, the wastewater sludge is a better raw material than commercial Soya medium for the biopesticides and enzyme production. The maximum entomotoxicity and protease activity observed in the fermentor was 9,332 IU/microL and 4.58 IU/mL, respectively. The proteases produced by Bt were also characterised. Two types of proteases were detected; neutral proteases with pH optimum 7.0 and alkaline proteases with pH optimum 10-11. Further, two types of alkaline proteases were detected; one having a pH and temperature optimum at 10 and 50 degrees C while the other at 11 and 70 degrees C. The protease thermal stability was found to increase in the presence of CaCl2, indicating the proteases were metalloproteases.

PMID: 12479478 [PubMed - indexed for MEDLINE]

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Correction 25.Sep.2006 01:38

strend

I, strend, was the author of the submission and not Tyagi RDS I submitted the "Simultaneous production of biopesticide and alkaline proteases by Bacillus thuringiensis using sewage sludge as a raw material." where he was one of the authors.

I am sorry, but I read the instructions wrong and appologize if I have committed a crime. This is not, now was my intention.
I only want SOMEONE to start connecting the dots.

Most Sincerely,

S Trend